This second edition has been extensively revised, updated, and enlarged to include new material covering the most recent topics and applications in this...
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Résumé
This second edition has been extensively revised, updated, and enlarged to include new material covering the most recent topics and applications in this fast-moving field. The book contains stateof-the-art protocols and applications on all aspects of Peptide Nucleic Acids (PNA) and in addition describes the theory and history of PNA. Concepts are explained clearly and in practical terms and each chapter contains concise background information. Written by leading experts in the field, the book is an invaluable contribution to this novel and exciting area. An important reference text and laboratory manual for all molecular biologists.
Sommaire
An Introduction to PNA
PNA Synthesis by Boc Chemistry
Synthesis of PNA Oligomers by Fmoc Chemistry
Thermodynamics of PNA Interactions
Peptide Nucleic Acid Targeting of Double-Stranded DNA
PNA Beacons and Fluoroprobes
Vector Tagging via PNA
PCR Clamping
PNA Directed Genome Rare Cutting : New Developements
PNA Openers for Duplex DNA : Basic Facts, Fine Tuning and Emerging Applications
Combined Use of PNA and DNA for Fluorescence IN Situ Hybridation (FISH)